Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)


  • Product nameDonkey Anti-Sheep IgG H&L (DyLight® 650)
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 650)
  • Target speciesSheep
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • ConjugationDyLight® 650. Ex: 654nm, Em: 673nm


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesDyLight® 650 replaces DyLight® 649.
  • Research areas


Our Abpromise guarantee covers the use of ab96942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to
ICC/IF 1/50 - 1/500.

Donkey Anti-Sheep IgG H&L (DyLight® 650) images

  • ab62116 staining MAP1LC3A in mouse splinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1 µg/ml in PBS/2% FBS) for 24 hour at 4°C. A DyLight® 650-conjugated donkey anti-sheep IgG H&L (ab96942) (1/1000) was used as the secondary antibody.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)

ab96942 has not yet been referenced specifically in any publications.

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Merci de nous avoir contactés.

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Merci de votre réponse.

Effectivement, c’est le ab96942 qui n’est pas préabsorbé. Je suis désolée que j’ai confondue les numéros de catalogue.

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Merci de nous avoir contacté. Je suis désolée d'apprendre que vous n'êtes pas satisfaites avec un de nos anticorps.
Effectivement l'anticorps ab6167 est un anticorps non pré-absorbé. Ceci veux dire que la réactivité contre des autres immunoglobuli...

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