Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)

Overview

  • Product nameDonkey Anti-Sheep IgG H&L (DyLight® 650)
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 650)
  • Target speciesSheep
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • ConjugationDyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesDyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
ICC/IF 1/50 - 1/500.

Donkey Anti-Sheep IgG H&L (DyLight® 650) images

  • ab62116 staining MAP1LC3A in mouse splinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1 µg/ml in PBS/2% FBS) for 24 hour at 4°C. A DyLight® 650-conjugated donkey anti-sheep IgG H&L (ab96942) (1/1000) was used as the secondary antibody.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)

ab96942 has not yet been referenced specifically in any publications.

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Merci de nous avoir contactés.

Je suis heureuse de vous confirmer la mise en place de l'envoie d'une unité gratuite de l'ab6564. Je me permets de vous transférer le numéro de commande de ceci :1064663 . Vous recevrez prochainement un mail de...

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Merci de votre réponse.

Effectivement, c’est le ab96942 qui n’est pas préabsorbé. Je suis désolée que j’ai confondue les numéros de catalogue.

Nous avons un anticorps qui p...

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Merci de nous avoir contacté. Je suis désolée d'apprendre que vous n'êtes pas satisfaites avec un de nos anticorps.
Effectivement l'anticorps ab6167 est un anticorps non pré-absorbé. Ceci veux dire que la réactivité contre des autres immunoglobuli...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"