Donkey Anti-Sheep IgG H&L (FITC) (ab6896)

Overview

  • Product nameDonkey Anti-Sheep IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target speciesSheep
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Immunogen

    Full length native Sheep whole (purified).

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
Immunomicroscopy
Flow Cyt
ELISA
  • Application notesFlow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey Anti-Sheep IgG H&L (FITC) (ab6896)

    ab6896 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    I used this antibody to detect sheep anti tyrosine hydroxylase (TH) labelling of splenic nerves in mouse spleen sections along with rat anti-CD35 labelling of follicular dendritic cells (FDC) and rabbit anti PrP labelling of prion protein. It was diluted 1/200 for use on frozen sections that were fixed in acetone and blocked with serum for 30 mins at room temperature. I chose this antibody as I found it difficult to find secondary's that were anti-sheep. I was concerned that the FITC might bleach during confocal microscopy however I was able to scan the triple labelling without any bleaching occurring.

    Image is of red CD35+ FDC, blue PrP and green TH+ nerves detected using donkey anti-sheep_FITC
    Username

    Ms. Laura McCulloch

    Verified customer

    Submitted Oct 23 2013

    I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"