Overview

  • Product nameDonkey Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesSheep
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Conjugation notesHorseradish Peroxidase (HRP)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
WB 1/3000 - 1/15000.
ELISA 1/150000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

Donkey Anti-Sheep IgG H&L (HRP) images

  • IHC image of Histone H3 staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab128012, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6900, 1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.

References for Donkey Anti-Sheep IgG H&L (HRP) (ab6900)

This product has been referenced in:
  • Durcan PJ  et al. Identification of novel Kirrel3 gene splice variants in adult human skeletal muscle. BMC Physiol 14:11 (2014). WB ; Human . Read more (PubMed: 25488023) »
  • Ziolkowska B  et al. alpha-Synuclein expression in the brain and blood during abstinence from chronic alcohol drinking in mice. Neuropharmacology 54:1239-46 (2008). WB . Read more (PubMed: 18456291) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.

It is normal to have brown precipitate form for HRP conjugates. The antibody that remains in solution is functional and the brown pellet should be discarded.

Irecommend to spin down the vial and try the a...

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Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest pipetting up and down few times,vortexing the vial and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"