Overview

  • Product name
    Donkey Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target species
    Sheep
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
WB 1/3000 - 1/15000.
ELISA 1/150000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

Images

  • IHC image of Histone H3 staining in Human normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab128012, 10µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6900, 1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.

References

This product has been referenced in:
  • Svarcbahs R  et al. Inhibition of Prolyl Oligopeptidase Restores Spontaneous Motor Behavior in the a-Synuclein Virus Vector-Based Parkinson's Disease Mouse Model by Decreasing a-Synuclein Oligomeric Species in Mouse Brain. J Neurosci 36:12485-12497 (2016). Read more (PubMed: 27927963) »
  • Durcan PJ  et al. Identification of novel Kirrel3 gene splice variants in adult human skeletal muscle. BMC Physiol 14:11 (2014). WB ; Human . Read more (PubMed: 25488023) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest pipetting up and down few times,vortexing the vial and...

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Thank you for your inquiry.

It is normal to have brown precipitate form for HRP conjugates. The antibody that remains in solution is functional and the brown pellet should be discarded.

Irecommend to spin down the vial and try the a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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