Overview

  • Product name
    Donkey Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (HRP)
  • Target species
    Sheep
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Sheep IgG.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (primary)
WB 1/5000 - 1/50000. Colorimetric: 1/500 - 1/30000, Chemiluminescent: 1/10000 - 1/50000

References for Donkey Anti-Sheep IgG H&L (HRP) (ab97125)

This product has been referenced in:
  • Van de Bittner GC  et al. Nasal neuron PET imaging quantifies neuron generation and degeneration. J Clin Invest 127:681-694 (2017). WB . Read more (PubMed: 28112682) »
  • Koch F  et al. Metabolic Response to Heat Stress in Late-Pregnant and Early Lactation Dairy Cows: Implications to Liver-Muscle Crosstalk. PLoS One 11:e0160912 (2016). WB . Read more (PubMed: 27513961) »

See all 4 Publications for this product

Product Wall

Abreviews
Application
Western blot
Rat olfactory epithelium tissue lysate, 10ug/lane
Blocked overnight in 5% nonfat milk
GAP43 1/2000, 1hr in TBS+0.1%Tween
Donkey anti-sheep 1/5000 in 3.5%BSA/TBS+-.1%Tween
Lane1: GAP43 primary antibody, Lane 2: Secondary only negative control

There is a general high background to the secondary but seems to be improved with secondary incubation in BSA and extended washing after secondary incubation.
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Verified customer

Submitted Jul 14 2015

Gracias por su respuesta. Un anticuerpo secundario válido para usar con el primario ab64544 podría ser el ab97125: http://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Sheep-IgG-H-L-HRP-ab97125.html está conjugado con peroxidasa y es apropia...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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