Overview

  • Product nameDonkey Anti-Sheep IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Target speciesSheep
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • ConjugationTRITC. Ex: 547nm, Em: 572nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesTetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000. PubMed: 20888852
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References for Donkey Anti-Sheep IgG H&L (TRITC) (ab6897)

This product has been referenced in:
  • D'Suze G  et al. Apoptogenic peptides from Tityus discrepans scorpion venom acting against the SKBR3 breast cancer cell line. Toxicon 56:1497-505 (2010). ICC/IF . Read more (PubMed: 20888852) »

See 1 Publication for this product

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TRITC conjugated or Dylight 549 conjugated could be suggested to customer.

ab6897 is a correct product.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"