Anti-DOPA Decarboxylase antibody (ab113357)

Overview

  • Product nameAnti-DOPA Decarboxylase antibody
    See all DOPA Decarboxylase primary antibodies
  • Description
    Rabbit polyclonal to DOPA Decarboxylase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Dog, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human DOPA Decarboxylase.

  • Positive control
    • This antibody gave a positive signal in both Human and Mouse Brain tissue lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • FunctionCatalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
  • PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.
  • Involvement in diseaseDefects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.
  • Sequence similaritiesBelongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AADC antibody
    • Aromatic L Amino Acid Decarboxylase antibody
    • Aromatic-L-amino-acid decarboxylase antibody
    • DDC antibody
    • DDC_HUMAN antibody
    • DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
    • DOPA decarboxylase antibody
    see all

Anti-DOPA Decarboxylase antibody images

  • All lanes : Anti-DOPA Decarboxylase antibody (ab113357) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 24 kDa,28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-DOPA Decarboxylase antibody (ab113357)

ab113357 has not yet been referenced specifically in any publications.

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