Overview

  • Product nameAnti-DOPA Decarboxylase antibody
    See all DOPA Decarboxylase primary antibodies
  • Description
    Rabbit polyclonal to DOPA Decarboxylase
  • Tested applicationsSuitable for: ICC, IHC-Fr, IHC-P, WB, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    SDS denatured, recombinant bovine aromatic L-amino acid decarboxylase expressed in E. coli and purified from inclusion bodies

Properties

Applications

Our Abpromise guarantee covers the use of ab3905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20374418
IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
  • PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.
  • Involvement in diseaseDefects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.
  • Sequence similaritiesBelongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AADC antibody
    • Aromatic L Amino Acid Decarboxylase antibody
    • Aromatic-L-amino-acid decarboxylase antibody
    • DDC antibody
    • DDC_HUMAN antibody
    • DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
    • DOPA decarboxylase antibody
    see all

Anti-DOPA Decarboxylase antibody images

  • ab3905 staining DOPA Decarboxylase in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was paraformaldehyde fixed, then blocked with 5% serum for 1 hour at 25°C. Primary anitbody used at a 1/500 dilution and incubated for 16 hours at 25°C. The secondary was used at 1/200 goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green).

    See Abreview

  • ab3905 staining DOPA Decarboxylase in mouse midbrain tissue sections by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 1% serum for 30 minutes at 24°C followed by incubation with the primary antibody, at a 1/250 dilution, for 16 hour at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG (green) was used as secondary antibody at a 1/250 dilution.

    See Abreview

References for Anti-DOPA Decarboxylase antibody (ab3905)

This product has been referenced in:
  • Wienecke J  et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014). Read more (PubMed: 25186745) »
  • Panayotis N  et al. Morphological and functional alterations in the substantia nigra pars compacta of the Mecp2-null mouse. Neurobiol Dis 41:385-97 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 20951208) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Fruit fly (Drosophila melanogaster) Cell (central nervous system (ventral nerve cord))
Specification central nervous system (ventral nerve cord)
Permeabilization Yes - 0.2% Triton-X
Fixative Formaldehyde
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shinya yamamoto

Verified customer

Submitted Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Nov 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Midbrain section)
Specification Midbrain section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
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Abcam user community

Verified customer

Submitted Mar 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% triton x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Aug 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (young postnatal mouse brain)
Specification young postnatal mouse brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain slices (40um thick sections))
Specification Brain slices (40um thick sections)
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Mar 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"