Overview

  • Product name
    Anti-DOPA Decarboxylase antibody
    See all DOPA Decarboxylase primary antibodies
  • Description
    Rabbit polyclonal to DOPA Decarboxylase
  • Tested applications
    Suitable for: ICC, IHC-Fr, IHC-P, WB, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    SDS denatured, recombinant bovine aromatic L-amino acid decarboxylase expressed in E. coli and purified from inclusion bodies

Properties

Applications

Our Abpromise guarantee covers the use of ab3905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20374418
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
  • Pathway
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.
  • Involvement in disease
    Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.
  • Sequence similarities
    Belongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AADC antibody
    • Aromatic L Amino Acid Decarboxylase antibody
    • Aromatic-L-amino-acid decarboxylase antibody
    • DDC antibody
    • DDC_HUMAN antibody
    • DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
    • DOPA decarboxylase antibody
    see all

Images

  • Anti-DOPA Decarboxylase antibody (ab3905) + Mouse cerebellum lysate at 0.2 mg/ml

    Performed under reducing conditions.
  • Anti-DOPA Decarboxylase antibody (ab3905) + Rat adrenal medulla lysate
  • ab3905 staining DOPA Decarboxylase in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was paraformaldehyde fixed, then blocked with 5% serum for 1 hour at 25°C. Primary anitbody used at a 1/500 dilution and incubated for 16 hours at 25°C. The secondary was used at 1/200 goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green).

    See Abreview

  • ab3905 staining DOPA Decarboxylase in mouse midbrain tissue sections by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 1% serum for 30 minutes at 24°C followed by incubation with the primary antibody, at a 1/250 dilution, for 16 hour at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG (green) was used as secondary antibody at a 1/250 dilution.

    See Abreview

References

This product has been referenced in:
  • Xenias HS  et al. Are striatal tyrosine hydroxylase interneurons dopaminergic? J Neurosci 35:6584-99 (2015). IHC ; Mouse . Read more (PubMed: 25904808) »
  • Wienecke J  et al. Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines. J Neurosci 34:11984-2000 (2014). Read more (PubMed: 25186745) »

See all 12 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Fruit fly (Drosophila melanogaster) Cell (central nervous system (ventral nerve cord))
Specification
central nervous system (ventral nerve cord)
Permeabilization
Yes - 0.2% Triton-X
Fixative
Formaldehyde
Username

shinya yamamoto

Verified customer

Submitted Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Midbrain section)
Specification
Midbrain section
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (young postnatal mouse brain)
Specification
young postnatal mouse brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain slices (40um thick sections))
Specification
Brain slices (40um thick sections)
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Mar 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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