Overview

  • Product name
    Anti-Dopamine antibody [2B11]
    See all Dopamine primary antibodies
  • Description
    Mouse monoclonal [2B11] to Dopamine
  • Specificity
    The affinity of this antibody for dopamine-BSA is 10 nM and for noradrenaline-BSA is 100 nM. Affinity for unconjugated catecholamines(dopamine, noradrenaline, adrenaline) is lower (approx. 100 µM). At 1 uM, this antibody inhibits dopamine binding to membrane receptor from human corpus striatum. Cross-reactivity (%): Dopamine = 100% Noradrenaline = <15% Adrenaline = <10% Normetanephrine = 0% 3-methoxy-tyramine = 0% L-DOPA = 0% Homovanillic acid = 0% Vanillic acid = 0% Vanillylmandelic acid = 0% 4-hydroxy-3-methoxyphenyl glycol = 0% 3,4-dihydroxyphenylacetic acid = 0% Tyramine = 0% beta-phenylethylamine = 0% Amphetamine = 0%
  • Tested applications
    Suitable for: Blocking, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Dopamine conjugated to Bovine Serum Albumin (BSA). Immunogen = Dopamine conjugated to bovine serum albumin.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    2B11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • Cellular localization
    Secreted
  • Alternative names
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

Anti-Dopamine antibody [2B11] images

  • ab1001 staining human adrenal tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 1% serum for 30 minutes at 22°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 30 minutes at 22°C.  A HRP conjugated goat anti-mouse antibody diluted 1/400 was used as the secondary.

    See Abreview

References for Anti-Dopamine antibody [2B11] (ab1001)

This product has been referenced in:
  • Rowell C  et al. Chemoprevention of breast cancer, proteomic discovery of genistein action in the rat mammary gland. J Nutr 135:2953S-2959S (2005). Read more (PubMed: 16317154) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adrenal)
Specification
adrenal
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adrenal)
Specification
adrenal
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2007

Thank you for sending this enquiry because it helped us to find a typographical error in our data sheet. The affinity of this antibody for dopamine-BSA should be 10 nM or 10(-8) M (the data sheet says 10 mM), and the affinity for noradrenaline-BSA shou...

Read More

This antibody should work in IHC on paraformaldehyde-fixed tissue without glutaraldehyde. However, we do not have any information on how well this antibody will work on macaca brain sections.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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