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Full length protein corresponding to Dopamine conjugated to Bovine Serum Albumin (BSA). Immunogen = Dopamine conjugated to bovine serum albumin.
Our Abpromise guarantee covers the use of ab1001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab1001 staining human adrenal tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 1% serum for 30 minutes at 22°C. The primary antibody was diluted 1/1000 and incubated with the sample for 30 minutes at 22°C. A HRP conjugated goat anti-mouse antibody diluted 1/400 was used as the secondary.