• Product nameAnti-Dopamine antibody [2B11]
    See all Dopamine primary antibodies
  • Description
    Mouse monoclonal [2B11] to Dopamine
  • SpecificityThis antibody detects catecholamine Affinity of this antibody: Kd = 10-8 for DA-BSA Kd = 10-7 for NA-BSA Kd = 10-4 for unconjugated DA
  • Tested applicationsSuitable for: ICC, Neutralisingmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to BSA.

  • General notes

    Detection of catecholamine-secreting cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesDetection of catecholamine-secreting cells.
  • ClonalityMonoclonal
  • Clone number2B11
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas


Our Abpromise guarantee covers the use of ab8281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.


  • RelevanceDopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • Cellular localizationSecreted
  • Alternative names
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

References for Anti-Dopamine antibody [2B11] (ab8281)

This product has been referenced in:
  • Baldrich E & Muñoz FX Carbon nanotube wiring: a tool for straightforward electrochemical biosensing at magnetic particles. Anal Chem 83:9244-50 (2011). Read more (PubMed: 22040046) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Leukemia cells)
Permeabilization Yes - 0.5% Triton-X100
Specification Leukemia cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jun 29 2017

Thank you for your enquiry. Yes, since the primary antibody was raised in mouse, this secondary will recognize it. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Dopamine conjugated with BSA has been used as the immunogen. However, the antibody is not conjugated.

According to literature data dopamine cross-links to some intracellular component during fixation/permeabilization and it remains inside the cell.

This antibody was raised against human dopamine, cross reactivity with other species has not yet been tested.

1% BSA solutions should be uses as normal as a blocking agent in IHC for both the primary and the secondary antibody. The immunogen was a dopamine-BSA conjugate, BSA faciltates the immune response.

This antibody is a mouse monoclonal (IgG1) to human dopamine, not to the D-2 receptor. As far as we are aware, the antibody has not yet been tested in mouse.