Overview

  • Product name
    Anti-Dopamine antibody [2B11]
    See all Dopamine primary antibodies
  • Description
    Mouse monoclonal [2B11] to Dopamine
  • Specificity
    This antibody detects catecholamine Affinity of this antibody: Kd = 10-8 for DA-BSA Kd = 10-7 for NA-BSA Kd = 10-4 for unconjugated DA
  • Tested applications
    Suitable for: ICC, Neutralisingmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to BSA.

  • General notes


    Detection of catecholamine-secreting cells.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    Detection of catecholamine-secreting cells.
  • Clonality
    Monoclonal
  • Clone number
    2B11
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.

Target

  • Relevance
    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • Cellular localization
    Secreted
  • Alternative names
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

References

This product has been referenced in:
  • Baldrich E & Muñoz FX Carbon nanotube wiring: a tool for straightforward electrochemical biosensing at magnetic particles. Anal Chem 83:9244-50 (2011). Read more (PubMed: 22040046) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Leukemia cells)
Permeabilization
Yes - 0.5% Triton-X100
Specification
Leukemia cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 29 2017

Thank you for your enquiry. Yes, since the primary antibody was raised in mouse, this secondary will recognize it. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Dopamine conjugated with BSA has been used as the immunogen. However, the antibody is not conjugated.

According to literature data dopamine cross-links to some intracellular component during fixation/permeabilization and it remains inside the cell.

This antibody was raised against human dopamine, cross reactivity with other species has not yet been tested.

1% BSA solutions should be uses as normal as a blocking agent in IHC for both the primary and the secondary antibody. The immunogen was a dopamine-BSA conjugate, BSA faciltates the immune response.

This antibody is a mouse monoclonal (IgG1) to human dopamine, not to the D-2 receptor. As far as we are aware, the antibody has not yet been tested in mouse.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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