Overview

  • Product name
  • Description
    Rabbit polyclonal to Dopamine
  • Specificity
    Using a conjugate Dopamine-Glutaraldehyde-bovine serum albumin, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
    G-BSA-conjugateCross-reactivity ratio (a)
    Dopamine1
    Dopamine(b)1/2.5
    Noradrenaline1/159
    L-DOPA, Octopamine1/400
    Tyramine, Adrenalin, unconjugated Dopamine 1/>5,000
    (a): Dopamine-G-BSA concentration/unconjugated or conjugated catecholamine concentration at half displacement (b): Non-reduced conjugate
  • Tested applications
    Suitable for: ICC, ICC/IF, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule by a Glutaraldehyde linker.

  • General notes

    The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Primary antibody notes
    The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. Glutharaldehyde perfusion fixation (see recommended protocol).
ELISA 1/5000.

Target

  • Relevance
    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • Cellular localization
    Secreted
  • Alternative names
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

Images

  • ab6427 at 1/5000 staining human stem cells (differentiated to neurons) by ICC. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 2 hours. A PE conjugated goatanti-rabbit antibody was used as the secondary.

    See Abreview

  • ab6427 staining dopamine in PC12 cells treated with ghrelin (rat) (ab120231), by ICC/IF. Decrease in dopamine expression correlates with increased concentration of ghrelin, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120231 (ghrelin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6427 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Wu SF  et al. Dopamine modulates hemocyte phagocytosis via a D1-like receptor in the rice stem borer, Chilo suppressalis. Sci Rep 5:12247 (2015). Read more (PubMed: 26179416) »
  • Forati E  et al. Neurotransmitter Specific, Cellular-Resolution Functional Brain Mapping Using Receptor Coated Nanoparticles: Assessment of the Possibility. PLoS One 10:e0145852 (2015). Read more (PubMed: 26717196) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

ab6427 is not guaranteed for use in immunohistochemistry. However, ab1001 (http://www.abcam.com/ab1001) is guaranteed to work in immunohistochemistry and gave good results when fixed with formalin/PFA in paraffi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (stem cells differentiated to neurons)
Specification
stem cells differentiated to neurons
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2
Username

Abcam user community

Verified customer

Submitted May 29 2007

We have additional information about capturing the DA as follows: After discussion with our research team, we think that the better way is to bind DA on your tissue via glutaraldehyde (0.1 to 1%). - Double bounds are reduced by adding of sodium bor...

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Thank you for your enquiry. For Immunohistological studies with dopamine antibodies, fixation with glutaraldehyde is required. Please find below a protocol. Perfusion protocol for adult male Sprague-Dawley (weight around 0.5 kg) 1- The animals ca...

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The pump that was used is a peristaltic pump with a flow of 150-300ml/minute. GILSON supply this pump type.

This antibody is known to work with 3-5% glutaraldehyde perfusion. Protocol is as follows - Perfusion : perfuse intracardially through the aorta using a pump with the following solutions : solution A (30ml) : 200-300ml/min solution B (500ml) : 200-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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