Overview

  • Product nameAnti-Dopamine antibody
    See all Dopamine primary antibodies
  • Description
    Rabbit polyclonal to Dopamine
  • Specificityab8888 targets conjugated dopamine, it does not recognize free dopamine.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICCmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Dopamine conjugated to Bovine Serum Albumin (BSA).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, ddH20
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Fixation of tissue for use with this antibody should be done with gluteraldehyde. The use of paraformaldehyde in conjunction with gluteraldehyde may improve staining results.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • RelevanceDopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • Cellular localizationSecreted
  • Alternative names
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

Anti-Dopamine antibody images

  • ab8888 at 1/500 staining human adrenal tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum before incubation with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References for Anti-Dopamine antibody (ab8888)

This product has been referenced in:
  • Simo L  et al. Evidence for d1 dopamine receptor activation by a paracrine signal of dopamine in tick salivary glands. PLoS One 6:e16158 (2011). IHC-Fr . Read more (PubMed: 21297964) »
  • Prandovszky E  et al. The neurotropic parasite toxoplasma gondii increases dopamine metabolism. PLoS One 6:e23866 (2011). IHC-P . Read more (PubMed: 21957440) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse midbrain)
Specification Mouse midbrain
Fixative 5% Glutaraldehyde
Blocking step Serum as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Aug 10 2012

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I fully understand the customers concerns and would be pleased to arrange afree of...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for contacting us.

You are quite correct. because of its size, the dopamine need to be attached to a macromolecule to be effectively visualized.

I hope this information is helpful to you. Please do not hesitate to co...

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Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse...

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Thank you for contacting us. I can confirm this antibody is suitable for conducting WB in mouse samples as the target (Dopamine) is a small molecule that does not vary between species. I hope this information is helpful to you. Please do not hesitate t...

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I have been contacted by the origonator of this product who has some additional information that you may be interested in. I have been contacted by the originator of this product who has some additional information that you may be interested in. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adrenal)
Specification adrenal
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenal)
Specification Adrenal
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2007

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"