Anti-Dopamine beta Hydroxylase antibody (ab19353)

Overview

  • Product nameAnti-Dopamine beta Hydroxylase antibody
    See all Dopamine beta Hydroxylase primary antibodies
  • Description
    Sheep polyclonal to Dopamine beta Hydroxylase
  • Tested applicationsSuitable for: ICC, WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Dopamine beta Hydroxylase aa 43-62.
    Sequence:

    SEPPESPFPYHIPLDPEGTL

  • General notesDetects human, bovine, rat and mouse DBH. It is not effective for immunohistochemistry in human brain

Properties

Applications

Our Abpromise guarantee covers the use of ab19353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/2500.
WB 1/5000. Detects a band of approximately 72 kDa.
ICC/IF 1/5000.
IHC-P 1/2000.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionConversion of dopamine to noradrenaline.
  • PathwayCatecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • Involvement in diseaseDefects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • Sequence similaritiesBelongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • dbh antibody
    • DBM antibody
    • Dopamine beta hydroxylase antibody
    • Dopamine beta monooxygenase antibody
    • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
    • Dopamine beta-monooxygenase antibody
    • DOPO_HUMAN antibody
    • OTTHUMP00000022501 antibody
    • Soluble dopamine beta-hydroxylase antibody
    see all

Anti-Dopamine beta Hydroxylase antibody images

  • Immunofluorescent staining of DBH in rat neuron cells using ab19353.

  • Immunofluorescent stain of DBH in rat brain tissue using ab19353.

References for Anti-Dopamine beta Hydroxylase antibody (ab19353)

This product has been referenced in:
  • Talavera-Adame D  et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). Read more (PubMed: 19364272) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain: Coronal sections of striatum and cortex)
Specification Brain: Coronal sections of striatum and cortex
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Verified customer

Submitted Jan 28 2016

DISCOUNT CODE: xxx
Expiration date: xxx
Value: xxx

I am very pleased to hear you would like to accept our offer and test ab19353 in IHC-Fr. This code will give you xxx off your next order before the expiration date. To redeem this off...

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Merci beaucoup de votre réponse et de vous avoir pris le temps de remplir le questionnaire.

Effectivement le point qui saute à l'œilen regardant leprotocole, est la méthode d'extraction de la protéine et pr&e...

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Je suis désolée d'apprendre que ab19353 ne vous donne pas de bons résultats en Western blot.

Veuillez trouver ci-jointlequestionnaire discuté au téléphone qui nous permet de regrouper le plus d'inform...

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Thank you for sending your protocol information. We haven't tested this antibody with perfusion-fixed or free floating tissue, but we generally recommend using an antigen retrieval method with formaldehyde-fixed tissue, as formaldehyde crosslinks p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"