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Full length native protein (purified) from adrenal medulla (Cow)
Our Abpromise guarantee covers the use of ab43868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-R||Use at an assay dependent dilution. PubMed: 20503062|
|IHC-FoFr||1/2000 - 1/4000. 1/2000 - 1/10000 (free-floating vibratome sections, ABC / DAB detection). Fixative: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over ~ 20 min. Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||1/400 - 1/800. 1/400 - 1/800, in PBS/0.3% Triton X-100.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"