Anti-Dopamine D2 Receptor antibody (ab150532)

Overview

  • Product name
    Anti-Dopamine D2 Receptor antibody
    See all Dopamine D2 Receptor primary antibodies
  • Description
    Rabbit polyclonal to Dopamine D2 Receptor
  • Specificity
    BLAST analysis of the peptide immunogen showed no homology with other human proteins.
  • Tested applications
    Suitable for: IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rabbit, Horse, Cow, Dog, Human, Aplysia, Bat
    Predicted to work with: Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Dopamine D2 Receptor. Synthetic peptide corresponding to a 16 amino acids from within the 3rd cytoplasmic domain of Human Dopamine Receptor D2L (NP_000786.1).

  • Positive control
    • Human brain tissue (neurons and glia).

Properties

Applications

Our Abpromise guarantee covers the use of ab150532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Involvement in disease
    Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue (neurons and glia) labeling Dopamine Receptor D2L with ab150532 at 10 µg/ml.

References

ab150532 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Striatum and VTA)
Specification
Striatum and VTA
Username

Abcam user community

Verified customer

Submitted Oct 27 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Aplysia Tissue sections (GANGLIA)
Specification
GANGLIA
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

Submitted Oct 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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