Anti-Dopamine D2 Receptor antibody (ab30743)

Overview

  • Product nameAnti-Dopamine D2 Receptor antibody
    See all Dopamine D2 Receptor primary antibodies
  • Description
    Goat polyclonal to Dopamine D2 Receptor
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Dopamine D2 Receptor aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32347, ab32348)

  • Positive control
    • This antibody gave a positive signal in the following Human adult normal tissue lysates: Brain, Liver, Heart and Skeletal Muscle This antibody gave a negative signal in the following Human adult normal tissue lysates: Testis, Kidney, Colon and Small Intestine

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab30743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51, 48 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionThis is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Involvement in diseaseDefects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

Anti-Dopamine D2 Receptor antibody images

  • All lanes : Anti-Dopamine D2 Receptor antibody (ab30743) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 6 : Human kidney tissue lysate - total protein (ab30203)
    Lane 7 : Human colon tissue lysate - total protein (ab30051)
    Lane 8 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 51, 48 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab30743 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30743, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931 Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Dopamine D2 Receptor antibody (ab30743)

ab30743 has not yet been referenced specifically in any publications.

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The MSDS files have been added to the online datasheets of these 3 antibodies (ab21218, ab32349, ab30743). You can find them now there or below:

ab21218: http://www.abcam.com/ps/Products/21/ab21218/Msds...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
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Dr. Sophie Pezet

Verified customer

Submitted Mar 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"