Anti-Dopamine D2 Receptor antibody (ab85367)
Key features and details
- Rabbit polyclonal to Dopamine D2 Receptor
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Dopamine D2 Receptor antibody
See all Dopamine D2 Receptor primary antibodies -
Description
Rabbit polyclonal to Dopamine D2 Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chimpanzee, Monkey -
Immunogen
Synthetic peptide corresponding to Human Dopamine D2 Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97357) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human brain; Human skeletal muscle; Rat Substantia nigra; Rat Dopaminergic nucleus.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa). |
Target
-
Function
This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. -
Involvement in disease
Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1813 Human
- Entrez Gene: 13489 Mouse
- Entrez Gene: 24318 Rat
- Omim: 126450 Human
- SwissProt: P14416 Human
- SwissProt: P61168 Mouse
- SwissProt: P61169 Rat
- Unigene: 73893 Human
see all -
Alternative names
- D(2) dopamine receptor antibody
- D2 dopamine receptor antibody
- D2DR antibody
see all
Images
-
All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml
Lane 1 : Rat Substantia Nigra Lysate
Lane 2 : Dopaminergic nucleus Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa (possible glycosylated form)
Exposure time: 4 minutes
Datasheets and documents
-
SDS download
-
Datasheet download
References (26)
ab85367 has been referenced in 26 publications.
- Williams MT et al. Cognitive and behavioral effects of whole brain conventional or high dose rate (FLASH) proton irradiation in a neonatal Sprague Dawley rat model. PLoS One 17:e0274007 (2022). PubMed: 36112695
- Hur KH et al. Methoxphenidine (MXP) induced abnormalities: Addictive and schizophrenia-related behaviours based on an imbalance of neurochemicals in the brain. Br J Pharmacol 178:3869-3887 (2021). PubMed: 33987827
- Yu J et al. Cabergoline Stimulates Human Endometrial Stromal Cell Decidualization and Reverses Effects of Interleukin-1β In Vitro. J Clin Endocrinol Metab 106:3591-3604 (2021). PubMed: 34260712
- Nakayama J et al. A zebrafish embryo screen utilizing gastrulation identifies the HTR2C inhibitor pizotifen as a suppressor of EMT-mediated metastasis. Elife 10:N/A (2021). PubMed: 34919051
- Kim YJ et al. An autophagy-related protein Becn2 regulates cocaine reward behaviors in the dopaminergic system. Sci Adv 7:N/A (2021). PubMed: 33608268