Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)

Overview

  • Product nameAnti-Dopamine Receptor D1 antibody [EP1560Y]
    See all Dopamine Receptor D1 primary antibodies
  • Description
    Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P21728

  • Positive control
    • WB: SH-SY5Y cell lysate, human fetal kidney, guinea pig brain, mouse and rat brain and kidney tissue lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEP1560Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
    • Tissue specificityDetected in caudate, nucleus accumbens and in the olfactory tubercle.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
    • Information by UniProt
    • Database links
    • Alternative names
      • D(1A) dopamine receptor antibody
      • D1A dopamine receptor antibody
      • DADR antibody
      • Dopamine D1 receptor antibody
      • dopamine receptor D1 antibody
      • DR D1 antibody
      • DR D1A antibody
      • DRD 1 antibody
      • DRD 1A antibody
      • DRD1 antibody
      • DRD1_HUMAN antibody
      • DRD1A antibody
      see all

    Anti-Dopamine Receptor D1 antibody [EP1560Y] images

    • All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat kidney tissue lysate
      Lane 5 : Guinea pig brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution

      Predicted band size : 49 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 49 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 49 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 49 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    References for Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)

    This product has been referenced in:
    • Brewer KL  et al. Dopamine D3 receptor dysfunction prevents anti-nociceptive effects of morphine in the spinal cord. Front Neural Circuits 8:62 (2014). WB ; Mouse . Read more (PubMed: 24966815) »
    • Mizuta K  et al. The dopamine D1 receptor is expressed and facilitates relaxation in airway smooth muscle. Respir Res 14:89 (2013). WB, IHC-P ; Guinea pig, Human . Read more (PubMed: 24004608) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Brain
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Brain
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    Application Immunohistochemistry (Frozen sections)
    Sample Zebra finch Tissue sections (Area X (striatum) brain tissue)
    Permeabilization No
    Specification Area X (striatum) brain tissue
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Mr. Jonnathan Singh

    Verified customer

    Submitted Jun 24 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"