For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant rat Dopamine Receptor D1, carboxy terminus.
Our Abpromise guarantee covers the use of ab78021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500. Predicted molecular weight: 49 kDa.|
|IHC (PFA fixed)||Use at an assay dependent concentration.|
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded rat cordical tissue sections, labelling Dopamine Receptor D1 with ab78021 at a dilution of 1/500 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was done via citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a Goat anti-mouse poyclonal biotin conjugate at 1/300. Image of subventricular region of striatum shows appropriate positivitiy.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"