Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021)


  • Product nameAnti-Dopamine Receptor D1 antibody [SG2-D1a]
    See all Dopamine Receptor D1 primary antibodies
  • Description
    Mouse monoclonal [SG2-D1a] to Dopamine Receptor D1
  • Tested applicationsSuitable for: IHC-P, WB, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep
    Does not react with: Rabbit, Cow, Dog, Human, Pig
  • Immunogen

    Recombinant rat Dopamine Receptor D1, carboxy terminus.

  • Positive control
    • Mouse brain lysate; rat caudate tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.60
    Preservative: 0.1% Sodium azide
    Constituent: 0.0536% PBS

    Please note that some batches of ab78021 may contain 0.4M arginine. Please contact Scientific Support for further information.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityMonoclonal
  • Clone numberSG2-D1a
  • IsotypeIgG2b
  • Research areas


Our Abpromise guarantee covers the use of ab78021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/250 - 1/500. Predicted molecular weight: 49 kDa.
IHC (PFA fixed) Use at an assay dependent concentration.


  • FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
  • Tissue specificityDetected in caudate, nucleus accumbens and in the olfactory tubercle.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
  • Information by UniProt
  • Database links
  • Alternative names
    • D(1A) dopamine receptor antibody
    • D1A dopamine receptor antibody
    • DADR antibody
    • Dopamine D1 receptor antibody
    • dopamine receptor D1 antibody
    • DR D1 antibody
    • DR D1A antibody
    • DRD 1 antibody
    • DRD 1A antibody
    • DRD1 antibody
    • DRD1_HUMAN antibody
    • DRD1A antibody
    see all

Anti-Dopamine Receptor D1 antibody [SG2-D1a] images

  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded rat cordical tissue sections, labelling Dopamine Receptor D1 with ab78021 at a dilution of 1/500 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was done via citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a Goat anti-mouse poyclonal biotin conjugate at 1/300. Image of subventricular region of striatum shows appropriate positivitiy.

    See Abreview

  • Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 20 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • IHC image of ab78021 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab78021, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021)

ab78021 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample Rat Tissue lysate - whole (hippocampi total membrane fraction)
Specification hippocampi total membrane fraction
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Sunetra Jitkar

Verified customer

Submitted Feb 19 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (Hippocampus)
Specification Hippocampus
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Feb 10 2014

Thank you for confirming that information. I have organised for ab98790 to be sent out to you (Order number xxxxx, Purchase order numberFOCR xxxxx). As we have the Easter bank holidays this will unfortunately only reach you on Wednesday next week.
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Thank you for getting back to me.

I can arrange for a different secondary antibody to be sent to you. In order to organise this I will need the order numbers of ab78021 and ab97040, or the approximate delivery date and delivery address.

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Thank you for contacting us.

As discussed over the phone, the dilution factors stated on the datasheet are recommended starting points only. Depending on the sample you are using and the protocol being used you may need to increase or d...

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Thank you for contacting us. The protocol is, for the most part, standard. The instruction to not boil the samples is referring to the tendency of multi-pass membrane proteins to aggregate if boiled. To denature your samples before loading into the...

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