Our Abpromise guarantee covers the use of ab42114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Dopamine Receptor D3 peptide (ab128688).|
|IP||Use at an assay dependent dilution. 2 µl of this antibody will immunoprecpitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells.|