Overview

  • Product nameAnti-Dopamine Receptor D4 antibody
    See all Dopamine Receptor D4 primary antibodies
  • Description
    Rabbit polyclonal to Dopamine Receptor D4
  • SpecificityCross-reactivity: D4 Dopamine Receptor (176-185) 100% D4 Dopamine Receptor 60% D1 Dopamine Receptor (9-21) 0% D1 Dopamine Receptor 0% D2 Dopamine Receptor (272-282) 0% D2 Dopamine Receptor 0% D3 Dopamine Receptor (2-10) 0% D3 Dopamine Receptor 0% D5 Dopamine Receptor (23-35) 0% D5 Dopamine Receptor 0%
  • Tested applicationsSuitable for: ICC, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    D4 (Ac-176-185) covalently attached to a carrier protein.

  • Positive control
    • Whole rat brain IF/ICC: PC12 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab20424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10000.
WB 1/500. Detects a band of approximately 48-53 kDa (predicted molecular weight: 48 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 22155235

Target

  • FunctionDopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Its activity is mediated by G proteins which inhibit adenylyl cyclase.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Polyubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex: polyubiquitination does not lead to degradation of DRD4 protein.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • D(2C) dopamine receptor antibody
    • D(4) dopamine receptor (D(2C) dopamine receptor). antibody
    • D(4) dopamine receptor antibody
    • D4 dopamine receptor antibody
    • D4DR antibody
    • Dopamine D4 receptor antibody
    • DRD 4 antibody
    • Drd4 antibody
    • DRD4_HUMAN antibody
    see all

Anti-Dopamine Receptor D4 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat gastric tissue labelling Dopamine Receptor D4 with ab20424 at 1/1000. Dopamine Receptor D4 was not found in the gastric mucosa (e - left) but was observed in the gastric enteric plexus (f - right). Paraffin sections were baked at 60°C for 2 hours, then deparaffinized in xylene for 20 minutes. Slides were rehydrated in descending concentrations of alcohol and transferred from 70% alcohol solution to distilled water for rinsing. Before staining, slides were pretreated with 10 mM citric acid (pH 6.0) at 92–98°C for 10 min and incubated in 10% normal serum and 0.1% Triton X-100 for 30 min. The sections were then incubated with the primary antibody, followed by the appropriate secondary antibody. Sections were washed in PBS and mounted in 90% glycerin PBS.

  • ab20424 stained PC12 cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20424 at 1/100) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

References for Anti-Dopamine Receptor D4 antibody (ab20424)

This product has been referenced in:
  • Wang Q  et al. Cellular localization of dopamine receptors in the gastric mucosa of rats. Biochem Biophys Res Commun 417:197-203 (2012). IHC-P ; Rat . Read more (PubMed: 22155235) »
  • Krishnan B  et al. Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced long-term potentiation in the rat amygdala following cocaine withdrawal. Eur J Neurosci 31:1027-42 (2010). Read more (PubMed: 20377617) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab20424.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"