Anti-Dopamine Receptor D5 antibody (ab40656)

Overview

  • Product nameAnti-Dopamine Receptor D5 antibody
    See all Dopamine Receptor D5 primary antibodies
  • Description
    Rabbit polyclonal to Dopamine Receptor D5
  • SpecificityCross-reacts with D5 Dopamine Receptor(Ac23-35) 100%, D5 Dopamine Receptor 80%. Does not cross react with other dopamine receptors (D1, D2, D3, D4). This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections, this includes cortex layer 5 and the parafascicular nucleus.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Human, Gorilla
  • Immunogen

    Synthetic peptide: AcGNAVGGSAGAPP conjugated to KLH, corresponding to amino acids 23-35 of Dopamine Receptor D5

  • Positive control
    • Whole rat brain homogenate rat brain sections

Properties

Applications

Our Abpromise guarantee covers the use of ab40656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2500.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/200. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). Blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

Target

  • FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
  • Tissue specificityNeuron-specific, localized primarily within limbic regions of the brain.
  • Involvement in diseaseDefects in DRD5 are a cause of benign essential blepharospasm (BEB) [MIM:606798]. BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • D(1B) dopamine receptor antibody
    • D(5) dopamine receptor antibody
    • D1 beta dopamine receptor antibody
    • D1B dopamine receptor antibody
    • D1beta dopamine receptor antibody
    • D5 dopamine receptor antibody
    • DBDR antibody
    • Dopamine D5 receptor antibody
    • Dopamine receptor D1B antibody
    • dopamine receptor D5 antibody
    • DRD 1B antibody
    • DRD 5 antibody
    • DRD1B antibody
    • DRD1L2 antibody
    • DRD5 antibody
    • DRD5_HUMAN antibody
    • MGC10601 antibody
    see all

Anti-Dopamine Receptor D5 antibody images

  • ICC/IF image of ab40656 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40656, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Dopamine Receptor D5 antibody (ab40656)

ab40656 has not yet been referenced specifically in any publications.

Product Wall

All our antibodies are for research purposes only and not for diagnostic or therapeutic ends. We therefore mainly test our products in in vitro application such as WB and IHC. All four antibodies mentioned have not been tested in vivo however this does...

Read More

Thank you for your enquiry.

I am sorry to confirm that regrettablythese lot numbers for these antibodies are no longer available. These are very old lot numbers.

Should you have any further questions, please do not hesitate to con...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Spleen cells)
Specification Spleen cells
Preparation Cell harvesting/tissue preparation method: Spleen cell suspensions were obtained by gently grinding tissue into RPMI 1640 culture medium (Sigma-Aldrich, Steinheim, Germany) under sterile conditions. Mononuclear cells were separated by Ficoll–Hypaque density gradient (1.083 g/ml) centrifugation. The mononuclear cells concentrated at the surface were collected and washed twice in RPMI 1640 medium. After cell counting, 1 x 10 6 cells/sample were stained with the corresponding antibodies.
Sample buffer: RPMI 1640 culture medium (Sigma-Aldrich, Steinheim, Germany)
Fixation No fixation
Permeabilization No
Gating Strategy Mononuclear Cells
Username

Abcam user community

Verified customer

Submitted May 27 2011

Application Western blot
Sample Rat Cell lysate - whole cell (Neuron)
Loading amount 50 µg
Specification Neuron
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"