Anti-Dopamine Transporter antibody [hDAT-NT] (ab5990)

Overview

  • Product name
    Anti-Dopamine Transporter antibody [hDAT-NT]
    See all Dopamine Transporter primary antibodies
  • Description
    Rat monoclonal [hDAT-NT] to Dopamine Transporter
  • Specificity
    This antibody recognizes the N-terminus of the dopamine transporter (DAT-NT) in mice and humans.
  • Tested applications
    Suitable for: IHC-P, ICC, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein corresponding to Dopamine Transporter. Fusion protein containing the dopamine transporter N-terminal amino acids 1-66.

  • Positive control
    • IHC-P: Mouse brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab5990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/1000.
IHC-Fr 1/500.

Target

  • Function
    Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Involvement in disease
    Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
  • Sequence similarities
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DA transporter antibody
    • DAT 1 antibody
    • DAT antibody
    • DAT1 antibody
    • Dopamine transporter 1 antibody
    • Dopamine transporter antibody
    • PKDYS antibody
    • SC6A3_HUMAN antibody
    • SLC6A3 antibody
    • Sodium dependent dopamine transporter antibody
    • Sodium-dependent dopamine transporter antibody
    • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
    • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
    • Solute carrier family 6 member 3 antibody
    • Variable number tandem repeat (VNTR) antibody
    see all

Anti-Dopamine Transporter antibody [hDAT-NT] images

  • ab5990 staining of dopamine transporter in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A Biotin-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

References for Anti-Dopamine Transporter antibody [hDAT-NT] (ab5990)

This product has been referenced in:
  • Zhou Q  et al. Impairment of PARK14-dependent Ca(2+) signalling is a novel determinant of Parkinson's disease. Nat Commun 7:10332 (2016). ICC ; Mouse . Read more (PubMed: 26755131) »
  • Postupna NO  et al. Flow cytometry analysis of synaptosomes from post-mortem human brain reveals changes specific to Lewy body and Alzheimer's disease. Lab Invest 94:1161-72 (2014). Flow Cyt . Read more (PubMed: 25068655) »

See all 8 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted May 16 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Karine Thibault

Verified customer

Submitted Aug 19 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat dopaminergic neuron)
Specification
rat dopaminergic neuron
Fixative
Formaldehyde
Permeabilization
No
Blocking step
saponin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2013

Thank you for contacting us.

ab104224
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I am very pleased to hear that your customer would like to accept our offer and test ab104224 in IHC-Fr. To redeem this offer, plea...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your enquiry,.

We recommend that antibodies should be stable for at least 1 year at the recommended storage temperature. This can be much longer for frozen antibodies. However, please noteour Abpromise guarantee is provided for...

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Thank you for contacting us.  We have three Dopamine Transporter antibodies that will be guaranteed for use in WB on rat samples: ab5981, ab5990, and ab18548.  Please find their datasheets attached.  I would be happy to help you further if you have any...

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Thank you for your enquiry. I regret to inform you that blocking peptides are not available for these 3 antibodies. If you have any additional questions, please don't hesitate to contact us again.

Thank you for your enquiry. Are looking for a dopamine transporter antibody or a dopamine antibody? You indicated ab5990 - Rat monoclonal to Dopamine Transporter - which is suitable for use in ICC but has not yet been tested for cross-reactivity with s...

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I'm sorry to hear you are having a problem with ab5990. Rat DAT contains various carbohydrate moieties and neuramnidase and N-Glycanase treatment reduces it size to ~55kD, so I am wondering if this could be the reason why you see a lower MW band. T...

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