Overview

  • Product nameAnti-Doppel antibody
  • Description
    Rabbit polyclonal to Doppel
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    TQAANQGEF

    , corresponding to amino acids 112-120 of Human Doppel.

  • Positive control
    • Mouse spleen 100k x g supernatant for Western blot.

Properties

Applications

Our Abpromise guarantee covers the use of ab23701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

Anti-Doppel antibody images

  • All lanes : Anti-Doppel antibody (ab23701) at 2 µg/ml

    Lane 1 : 27ug mouse spleen 100k x g supernatant.
    Lane 2 : 54ug mouse spleen 100k x g supernatant.


    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa (possible glycosylated form).

References for Anti-Doppel antibody (ab23701)

ab23701 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (CNS)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification CNS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"