The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.
Tissue specificityWidely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RBR family. RNF19 subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationMembrane. Cytoplasm > cytoskeleton > centrosome. Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
Ring IBR ring domain containing protein Dorfin antibody
Anti-DORFIN antibody images
Immunoprecipitation - DORFIN antibody (ab113276)
Detection of DORFIN by Western Blot of Immunprecipitate.
ab113276 at 1µg/ml staining DORFIN in HeLa whole cell lysate immunoprecipitated using ab113276 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-DORFIN antibody (ab113276)
has not yet been referenced specifically in any publications.
Publishing research using ab113276? Please let us know so that we can cite the reference in this datasheet.
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