Overview

  • Product nameAnti-DORFIN antibody
    See all DORFIN primary antibodies
  • Description
    Rabbit polyclonal to DORFIN
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 600-650 of Human DORFIN (NP_056250.3).

  • Positive control
    • HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab113276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.
    • Tissue specificityWidely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the RBR family. RNF19 subfamily.
      Contains 1 IBR-type zinc finger.
      Contains 2 RING-type zinc fingers.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationMembrane. Cytoplasm > cytoskeleton > centrosome. Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
    • Information by UniProt
    • Database links
    • Alternative names
      • AA032313 antibody
      • DKFZP566B1346 antibody
      • Dorfin antibody
      • Double ring finger protein antibody
      • Double ring-finger protein antibody
      • E3 ubiquitin-protein ligase RNF19A antibody
      • p38 antibody
      • p38 protein antibody
      • Ring finger protein 19 antibody
      • RING finger protein 19A antibody
      • Ring IBR ring domain containing protein Dorfin antibody
      • RN19A_HUMAN antibody
      • RNF19 antibody
      • RNF19A antibody
      • Ubce7ip2 antibody
      • UIP117 antibody
      • XYbp antibody
      see all

    Anti-DORFIN antibody images

    • Detection of DORFIN by Western Blot of Immunprecipitate.
      ab113276 at 1µg/ml staining DORFIN in HeLa whole cell lysate immunoprecipitated using ab113276 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    References for Anti-DORFIN antibody (ab113276)

    ab113276 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab113276.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"