Overview

  • Product nameAnti-DOS antibody
  • Description
    Rabbit polyclonal to DOS
  • Specificityab121378 shows specific reactivity against target antigen.
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 70-179 of Human DOS, isoform 2.

  • Positive control
    • Human kidney tissue. U-251 MG cell and Human Liver lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-DOS antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli. Recommended concentration of ab121378 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121378 at 1/100 dilution staining DOS in Human kidey tissue by Immunohistochemistry. This image shows cytoplasmic positivity in cells in tubules.

References for Anti-DOS antibody (ab121378)

ab121378 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"