Anti-Doublecortin antibody (ab113435)

Overview

  • Product nameAnti-Doublecortin antibody
    See all Doublecortin primary antibodies
  • Description
    Goat polyclonal to Doublecortin
  • Specificityab113435 is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Cat, Dog, Human, Pig
  • Immunogen

    Synthetic peptide:

    C-KTSANMKAPQS

    , corresponding to internal sequence amino acids 151-161 of Human Doublecortin (NP_835365.1; NP_835364.1; NP_001182482.1.), or amino acids 232-242 of Human Doublecortin (NP_000546.2).

  • Positive control
    • Mouse fetal Brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionSeems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • Tissue specificityHighly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • Involvement in diseaseDefects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • Sequence similaritiesContains 2 doublecortin domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBCN antibody
    • Dbct antibody
    • DC antibody
    • DCX antibody
    • DCX_HUMAN antibody
    • Doublecortex antibody
    • Doublin antibody
    • FLJ51296 antibody
    • Lis X antibody
    • Lis-X antibody
    • Lissencephalin X antibody
    • Lissencephalin-X antibody
    • Lissencephaly X linked antibody
    • Lissencephaly X linked doublecortin antibody
    • LISX antibody
    • Neuronal migration protein doublecortin antibody
    • OTTHUMP00000023859 antibody
    • OTTHUMP00000023860 antibody
    • OTTHUMP00000216315 antibody
    • OTTHUMP00000216316 antibody
    • SCLH antibody
    • XLIS antibody
    see all

Anti-Doublecortin antibody images

  • Anti-Doublecortin antibody (ab113435) at 0.01 µg/ml + Mouse Fetal Brain lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 49 kDa

References for Anti-Doublecortin antibody (ab113435)

ab113435 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Antwort. Ich habe Ihnen einen Anti-Doublecortin Antikoerper aus der Ziege herausgesucht. Click here (or use the following: http://www.abcam.com/index.html?datasheet=113435). Doublecortin färbt keine Glia -Zellen an (siehe ...

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Thank you for getting back to me with those details.
As I have mentioned previously, we have not performed any IHC experiments with this antibody, we would therefore not be sure of how well it would perform in this application.
You mentioned ...

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Thank you for contacting us and reporting the difficulty you have found in using the anti-Doublecortin antibody (ab113435) in IHC.
As discussed over the phone, we have not ourselves tested this antibody in IHC and have had no reports of our custom...

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Thank you very much for your interest in ab113435.

We do not supply samples of our antibodies and so therefore I am unable to send you an aliquot to try out and you are correct that ab18723 is a rabbit polyclonal antibody.

As ab113...

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Thank you for contacting Abcam.

We have not yet tested ab113435 in IHC on brain sections, therefore we do not have a protocol that has been worked out for this antibody, I have however attached the Abcam general IHC protocol. Also as it has n...

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