Immunohistochemistry (Frozen sections) abreview for Anti-Doublecortin antibody - Neuronal Marker

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult brain, dentate gyrus)
Specification
Adult brain, dentate gyrus
Fixative
Paraformaldehyde
Permeabilization
Yes - TBST 1x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% FCS 0.01% Tx-100 in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Jackson Anti-rabbit 711-165-152
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/500

Additional data

PubMed ID
20808958: View
Username

Abcam user community

Verified customer

Submitted Oct 12 2010

Sign up