Anti-Doublecortin antibody (ab54739)

Overview

  • Product nameAnti-Doublecortin antibody
    See all Doublecortin primary antibodies
  • Description
    Mouse monoclonal to Doublecortin
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tagged recombinant fragment, corresponding to amino acids 82-336 of human Doublecortin (O43602; AAH27925)

  • Positive control
    • Doublecortin transfected 293T cell lysate; recombinant Doublecortin protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab54739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 49 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionSeems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • Tissue specificityHighly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • Involvement in diseaseDefects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • Sequence similaritiesContains 2 doublecortin domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBCN antibody
    • Dbct antibody
    • DC antibody
    • DCX antibody
    • DCX_HUMAN antibody
    • Doublecortex antibody
    • Doublin antibody
    • FLJ51296 antibody
    • Lis X antibody
    • Lis-X antibody
    • Lissencephalin X antibody
    • Lissencephalin-X antibody
    • Lissencephaly X linked antibody
    • Lissencephaly X linked doublecortin antibody
    • LISX antibody
    • Neuronal migration protein doublecortin antibody
    • OTTHUMP00000023859 antibody
    • OTTHUMP00000023860 antibody
    • OTTHUMP00000216315 antibody
    • OTTHUMP00000216316 antibody
    • SCLH antibody
    • XLIS antibody
    see all

Anti-Doublecortin antibody images

  • Anti-Doublecortin antibody (ab54739) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 49 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Doublecortin antibody (ab54739) at 5 µg/ml

    Lane 1 : Doublecortin transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 49 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa (possible non-specific binding),38 kDa. We are unsure as to the identity of these extra bands.
  • ab54739 at 10 ug/ml staining Doublecortin in human Hela cells by Immunocytochemistry / Immunofluorescence.

References for Anti-Doublecortin antibody (ab54739)

ab54739 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (C57BL/6 (8w), Brain, perfusion fixed 4% FA, 10 um)
Specification C57BL/6 (8w), Brain, perfusion fixed 4% FA, 10 um
Fixative Acetone
Permeabilization Yes - 0.01% Tween 20
Blocking step PBS +1% BSA +1% ovalbumine +1% goat serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Aug 13 2009

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