Anti-Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544)

Overview

  • Product nameAnti-Doublecortin (phospho S28) antibody - Neuronal Marker
    See all Doublecortin primary antibodies
  • Description
    Rabbit polyclonal to Doublecortin (phospho S28) - Neuronal Marker
  • Tested applicationsSuitable for: IHC-FoFr, WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Doublecortin, phosphorylated at S28.

    (Peptide available as ab31511.)

  • Positive control
    • Mouse Adult Brain Whole Tissue Lysate and Mouse (0-days old) Brain Whole Tissue Lysate. Rat adult brain tissue.
  • General notes


    Doublecortin (DCX) undergoes a cycle of dephosphorylation and phosphorylation that regulates its binding to microtubules in growing neurites. The N-terminus serine-28 is a major site of phosphorylation for DCX; this was identified by Graham et al (2004). Single mutation of Ser-28 reduced but did not abolish phosphorylation; DCX is subject to complex multi-site phosphorylation

Properties

Applications

Our Abpromise guarantee covers the use of ab23544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23226105
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionSeems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
    • Tissue specificityHighly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
    • Involvement in diseaseDefects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
      Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
      Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
    • Sequence similaritiesContains 2 doublecortin domains.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DBCN antibody
      • Dbct antibody
      • DC antibody
      • DCX antibody
      • DCX_HUMAN antibody
      • Doublecortex antibody
      • Doublin antibody
      • FLJ51296 antibody
      • Lis X antibody
      • Lis-X antibody
      • Lissencephalin X antibody
      • Lissencephalin-X antibody
      • Lissencephaly X linked antibody
      • Lissencephaly X linked doublecortin antibody
      • LISX antibody
      • Neuronal migration protein doublecortin antibody
      • OTTHUMP00000023859 antibody
      • OTTHUMP00000023860 antibody
      • OTTHUMP00000216315 antibody
      • OTTHUMP00000216316 antibody
      • SCLH antibody
      • XLIS antibody
      see all

    Anti-Doublecortin (phospho S28) antibody - Neuronal Marker images

    • Immunofluorescent staining for Doublecortin (phospho S28) in the rat habenular nucleus using Rabbit polyclonal to Doublecortin (phospho S28). The staining pattern observed with ab23544 appears to be specific, however, the staining is not very strong or contrasted - this might be expected for detection of a modification protein in unstimulated tissue. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in free floating IHC. Primary antibody ab123544 was incubated overnight at 1/1000 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.

    References for Anti-Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544)

    This product has been referenced in:
    • Feltrin D  et al. Growth cone MKK7 mRNA targeting regulates MAP1b-dependent microtubule bundling to control neurite elongation. PLoS Biol 10:e1001439 (2012). ICC/IF ; Mouse . Read more (PubMed: 23226105) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Hipocampus)
    Specification Hipocampus
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 09 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Hippocampus)
    Specification Hippocampus
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 09 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain sections (30 um free floating))
    Specification Brain sections (30 um free floating)
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted Jan 07 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"