DoubleStain IHC Kit: G&M on human tissue (DAB & AP/Red) (ab183159)

Overview

  • Product name
    DoubleStain IHC Kit: G&M on human tissue (DAB & AP/Red)
  • Assay type
    Polymer Multiplex
  • Product overview

    The DoubleStain IHC kit (ab183159) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples. This kit has been tested in paraffin-embedded formalin-fixed tissues. However, this kit can be used on frozen specimens and freshly prepared monolayer cell smears.

     

    Double staining is a common method used in immunohistochemistry for the detection of two distinct antigens in a single tissue. Abcam’s DoubleStain IHC Kit supplies two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP polymer anti-Mouse IgG with two distinct substrates/chromogens, DAB (Brown) and Permanent Red (red). Simplified steps offer a convenient protocol as the enzyme conjugates are applied to the specimen sequentially. If only the anti-goat antigen is present only the Permanent Red (red) will be present and if only the anti-mouse antigen is present only the DAB (brown) will be present. The DoubleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding.

  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    Aqueous Mounting Medium 1 x 7ml 1 x 18ml 1 x 70ml
    DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 3ml
    DAB Substrate 1 x 12ml 2 x 15ml 1 x 70ml
    DoubleStain Block 1 x 6ml 1 x 18ml 1 x 60ml
    Goat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 1 x 14ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 0.7ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 70ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Protocols

References

ab183159 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183159.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up