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The DoubleStain IHC kit (ab183159) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples. This kit has been tested in paraffin-embedded formalin-fixed tissues. However, this kit can be used on frozen specimens and freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry for the detection of two distinct antigens in a single tissue. Abcam’s DoubleStain IHC Kit supplies two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP polymer anti-Mouse IgG with two distinct substrates/chromogens, DAB (Brown) and Permanent Red (red). Simplified steps offer a convenient protocol as the enzyme conjugates are applied to the specimen sequentially. If only the anti-goat antigen is present only the Permanent Red (red) will be present and if only the anti-mouse antigen is present only the DAB (brown) will be present. The DoubleStain Kit is a non-biotin system avoiding non-specific background due to endogenous biotin binding.
|Components||12 ml||36 ml||120 ml|
|Aqueous Mounting Medium||1 x 7ml||1 x 18ml||1 x 70ml|
|DAB Chromogen (20x)||1 x 1.5ml||1 x 2ml||1 x 3ml|
|DAB Substrate||1 x 12ml||2 x 15ml||1 x 70ml|
|DoubleStain Block||1 x 6ml||1 x 18ml||1 x 60ml|
|Goat AP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
|Mouse HRP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
|Permanent Red Activator (5x)||1 x 3ml||1 x 7.2ml||1 x 14ml|
|Permanent Red Chromogen (100x)||1 x 150µl||1 x 360µl||1 x 0.7ml|
|Permanent Red Substrate||1 x 15ml||2 x 18ml||1 x 70ml|
Our Abpromise guarantee covers the use of ab183159 in the following tested applications.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183159 has not yet been referenced specifically in any publications.