Recombinant Anti-DP1 antibody [EPR4959(2)] (ab124678)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4959(2)] to DP1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DP1 antibody [EPR4959(2)]
See all DP1 primary antibodies -
Description
Rabbit monoclonal [EPR4959(2)] to DP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human DP1 aa 350-450. The exact sequence is proprietary.
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Positive control
- Raji cell lysate; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4959(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis. -
Tissue specificity
Highest levels in muscle. Also expressed in brain, placenta, liver and kidney. Lower levels in lung and pancreas. Not detected in heart. -
Sequence similarities
Belongs to the E2F/DP family. -
Post-translational
modificationsPhosphorylation by E2F-1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7027 Human
- Entrez Gene: 21781 Mouse
- Entrez Gene: 361178 Rat
- Omim: 189902 Human
- SwissProt: Q14186 Human
- SwissProt: Q08639 Mouse
- Unigene: 79353 Human
- Unigene: 925 Mouse
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Alternative names
- DP 1 antibody
- DP1 antibody
- DRTF1 antibody
see all
Images
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ab124678 at 1/10 dilution staining DP1 in permeabilized HeLa cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).
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Anti-DP1 antibody [EPR4959(2)] (ab124678) at 1/10000 dilution + Raji cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124678 has been referenced in 3 publications.
- Guo T et al. KDM6B interacts with TFDP1 to activate P53 signaling in regulating mouse palatogenesis. Elife 11:N/A (2022). PubMed: 35212626
- Gao X et al. GeneChip expression profiling identified OLFML2A as a potential therapeutic target in TNBC cells. Ann Transl Med 10:274 (2022). PubMed: 35433966
- Wang H et al. Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma. J Exp Clin Cancer Res 39:216 (2020). PubMed: 33054826