Recombinant
RabMAb

Anti-DP1 antibody [EPR4959(2)] (ab124678)

Overview

  • Product name
    Anti-DP1 antibody [EPR4959(2)]
    See all DP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4959(2)] to DP1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human DP1.

  • Positive control
    • Raji cell lysate; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.
    • Tissue specificity
      Highest levels in muscle. Also expressed in brain, placenta, liver and kidney. Lower levels in lung and pancreas. Not detected in heart.
    • Sequence similarities
      Belongs to the E2F/DP family.
    • Post-translational
      modifications
      Phosphorylation by E2F-1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DP 1 antibody
      • DP1 antibody
      • DRTF1 antibody
      • DRTF1-polypeptide 1 antibody
      • E2F dimerization partner 1 antibody
      • E2F related transcription factor antibody
      • TFDP1 antibody
      • TFDP1_HUMAN antibody
      • Transcription factor Dp-1 antibody
      • Transcription factor sequence-specific, DRTF1 antibody
      see all

    Images

    • ab124678 at 1/10 dilution staining DP1 in permeabilized HeLa cells by Flow cytometry (red). Rabbit IgG negative control (green).
    • Anti-DP1 antibody [EPR4959(2)] (ab124678) at 1/10000 dilution + Raji cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 49 kDa (why is the actual band size different from the predicted?)

    References

    ab124678 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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