Overview

  • Product nameAnti-DP2 antibodySee all DP2 primary antibodies ...
  • Description
    Rabbit polyclonal to DP2
  • Specificityab26260 recognises TFDP2.
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: MIISTPQRLT SSGSVLIGSP YTPAPAMVTQ THIAEATGWV PGDRKRARKF, corresponding to a region within N terminal amino acids 1-50 of Human DP2

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab26260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.

Target

  • FunctionCan stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.
  • Tissue specificityHigh levels in heart and skeletal muscle. Also found in placenta, kidney, brain, lung and liver. The presence as well as the abundance of the different transcripts appear to vary significantly in different tissues and cell lines.
  • Sequence similaritiesBelongs to the E2F/DP family.
  • Post-translational
    modifications
    Ser-24 is probably phosphorylated by CDK2.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q14188 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DP2 antibody
    • E2F dimerization partner 2 antibody
    • TFDP2 antibody
    • TFDP2 antibody
    • TFDP2_HUMAN antibody
    • Transcription factor Dp 2 antibody
    • Transcription factor Dp 2 antibody
    • Transcription factor Dp-2 antibody
    see all

Anti-DP2 antibody images

  • Anti-DP2 antibody (ab26260) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50000 - 1/100000

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • Staining of human kidney tissue with ab26260 (4-8 ug/ml). Positive labelled epithelial cells of renal tubule are indicated with arrows. 400X magnification.

References for Anti-DP2 antibody (ab26260)

ab26260 has not yet been referenced specifically in any publications.

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