Overview

  • Product name
  • Description
    Rabbit polyclonal to DPAGT1
  • Specificity
    At least four isoforms of DPAGT1 are known to exist; ab117459 will recognize the two longest isoforms. ab117459 is predicted to not cross-react with UHRF1BP1.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to 17 amino acids within a region near the N terminal of Human DPAGT1 (NP_001373).

  • Positive control
    • Mouse kidney tissue lysate.
  • General notes
    Avoid repeated freeze thaw cycles and exposure to prolonged high temperatures.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117459 was purified by affinity chromatography via a peptide column.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab117459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration.
ICC Use a concentration of 2.5 µg/ml.

Target

  • Function
    Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.
  • Pathway
    Protein modification; protein glycosylation.
  • Involvement in disease
    Defects in DPAGT1 are the cause of congenital disorder of glycosylation type 1J (CDG1J) [MIM:608093]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
  • Sequence similarities
    Belongs to the glycosyltransferase 4 family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALG7 antibody
    • AU021132 antibody
    • CDG Ij antibody
    • CDG1J antibody
    • CMS13 antibody
    • CMSTA2 antibody
    • D11S366 antibody
    • DGPT antibody
    • Dolichyl phosphate (UDP N acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc 1 P transferase) antibody
    • Dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 antibody
    • DPAGT1 antibody
    • DPAGT2 antibody
    • G1PT antibody
    • GlcNAc 1 P transferase 1 antibody
    • GlcNAc-1-P transferase antibody
    • Gnpta antibody
    • GPT antibody
    • GPT_HUMAN antibody
    • H2afx antibody
    • N-acetylglucosamine-1-phosphate transferase antibody
    • UAGT antibody
    • UDP GlcNAc:dolichyl phosphate N acetylglucosaminephosphotransferase antibody
    • UDP N acetylglucosamine dolichyl phosphate N acetylglucosaminephosphotransferase 1 antibody
    • UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase antibody
    • UGAT antibody
    see all

Anti-DPAGT1 antibody images

  • Immunofluorescence of DPAGT1 in Human kidney tissue with ab117459 at 20 ug/mL.
  • All lanes : Anti-DPAGT1 antibody (ab117459) at 1 µg/ml

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 46 kDa
  • Immunohistochemical analysis of Human kidney tissue, staining DPAGT1 with ab117459 at 2.5 µg/ml.

References for Anti-DPAGT1 antibody (ab117459)

ab117459 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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