Anti-DPEP1/MDP antibody (ab121308)
Key features and details
- Rabbit polyclonal to DPEP1/MDP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-DPEP1/MDP antibody
See all DPEP1/MDP primary antibodies -
Description
Rabbit polyclonal to DPEP1/MDP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DPEP1/MDP aa 263-388.
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Positive control
- IHC: Human Plasma lysate; Human small intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 46 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. -
Sequence similarities
Belongs to the peptidase M19 family. -
Cellular localization
Apical cell membrane. Cell projection > microvillus membrane. Brush border membrane. - Information by UniProt
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Database links
- Entrez Gene: 1800 Human
- Omim: 179780 Human
- SwissProt: P16444 Human
- Unigene: 109 Human
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Alternative names
- Dehydropeptidase-I antibody
- Dipeptidase 1 (renal) antibody
- Dipeptidase 1 antibody
see all
Images
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All lanes : Anti-DPEP1/MDP antibody (ab121308) at 0.4 µg/ml
Lane 1 : Control - vector only transfected HEK293T lysate
Lane 2 : DPEP1 over-expression lysate - co-expressed with a C-terminal myc-DDY tag (3.1kDa)
Predicted band size: 46 kDa -
ab121308 at 1/2500 dilution staining DPEP1/MDP in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
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ab121308 at 1/2500 dilution staining DPEP1/MDP in Paraffin-embedded Human lymp node tissue by Immunohistochemistry. This shows low expresion as expected.
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ab121308 at 1/2500 dilution staining DPEP1/MDP in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab121308 has been referenced in 2 publications.
- Lau A et al. Dipeptidase-1 governs renal inflammation during ischemia reperfusion injury. Sci Adv 8:eabm0142 (2022). PubMed: 35108057
- Choudhury SR et al. Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver. Cell 178:1205-1221.e17 (2019). PubMed: 31442408