Anti-DPF2 antibody [2283C1a] (ab74519)

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Overview

  • Product name
    Anti-DPF2 antibody [2283C1a]
    See all DPF2 primary antibodies
  • Description
    Mouse monoclonal [2283C1a] to DPF2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment from internal sequence of human DPF2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab74519 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and then filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    2283C1a
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 44 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
  • Information by UniProt
  • Database links
    • Alternative names
      • Apoptosis response zinc finger protein antibody
      • BAF45D antibody
      • BRG1-associated factor 45D antibody
      • D4 antibody
      • D4 zinc and double PHD fingers family 2 antibody
      • Double PHD fingers 2 antibody
      • double PHD fingers family 2 antibody
      • DPF 2 antibody
      • DPF2 antibody
      • MGC10180 antibody
      • Protein requiem antibody
      • REQ antibody
      • REQU_HUMAN antibody
      • Requiem antibody
      • Requiem apoptosis response zinc finger antibody
      • UBI D4 antibody
      • ubi-d4 antibody
      • UBID 4 antibody
      • UBID4 antibody
      • zinc and double PHD fingers family 2 antibody
      • Zinc finger protein ubi d4 antibody
      • Zinc finger protein ubi-d4 antibody
      see all

    Images

    • Western blot - Anti-DPF2 antibody [2283C1a] (ab74519)
      Western blot - Anti-DPF2 antibody [2283C1a] (ab74519)
      Anti-DPF2 antibody [2283C1a] (ab74519) + Immunising recombinant protein

      Predicted band size: 44 kDa
      Observed band size: 39 kDa (why is the actual band size different from the predicted?)



      The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

    References

    ab74519 has not yet been referenced specifically in any publications.

    Publishing research using ab74519? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    Votes: Highest First

    ChIP abreview for Anti-DPF2 antibody [2283C1a]

     Poor
    Abreviews
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Jurkat Cells)
    Negative control
    Input
    Specification
    Jurkat Cells
    Detection step
    Other
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Positive control
    Abcam ab134942, which has worked for ChIP in the past
    Read More
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    Verified customer

    Submitted Nov 09 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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