The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3125 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Sequence similaritiesBelongs to the requiem/DPF family. Contains 1 C2H2-type zinc finger. Contains 2 PHD-type zinc fingers.