Overview

  • Product nameAnti-DPF2 antibody
    See all DPF2 primary antibodies
  • Description
    Rabbit polyclonal to DPF2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: MAAVVENVVK LLGEQYYKDA MEQCHNYNAR LCAERSVRLP FLDSQTGVAQ, corresponding to N terminal amino acids 1-50 of Human DPF2

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Cellular localizationNucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis response zinc finger protein antibody
    • BAF45D antibody
    • BRG1-associated factor 45D antibody
    • D4 antibody
    • D4 zinc and double PHD fingers family 2 antibody
    • double PHD fingers family 2 antibody
    • DPF 2 antibody
    • DPF2 antibody
    • MGC10180 antibody
    • Protein requiem antibody
    • REQ antibody
    • REQU_HUMAN antibody
    • Requiem antibody
    • Requiem apoptosis response zinc finger antibody
    • UBI D4 antibody
    • ubi-d4 antibody
    • UBID 4 antibody
    • UBID4 antibody
    • zinc and double PHD fingers family 2 antibody
    • Zinc finger protein ubi d4 antibody
    • Zinc finger protein ubi-d4 antibody
    see all

Anti-DPF2 antibody images

  • All lanes : Anti-DPF2 antibody (ab66647) at 0.3125 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-DPF2 antibody (ab66647)

ab66647 has not yet been referenced specifically in any publications.

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