Overview

  • Product name
  • Description
    Rabbit polyclonal to DPH2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 202-419 of Human DPH2 (UniProt: Q9BQC3).

  • Positive control
    • HeLa whole cell lysate; Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab152121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8.

Target

  • Function
    Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2.
  • Tissue specificity
    Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes.
  • Sequence similarities
    Belongs to the DPH1/DPH2 family. DPH2 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Diphthamide biosynthesis like protein 2 antibody
    • Diphthamide biosynthesis protein 2 antibody
    • Diphthamide biosynthesis protein 2 homolog like 2 antibody
    • Diphthamide biosynthesis protein 2 homolog-like 2 antibody
    • Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 antibody
    • Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 S. cerevisiae antibody
    • DPH 2 antibody
    • DPH 2 homolog antibody
    • DPH2 antibody
    • DPH2 homolog antibody
    • DPH2 homolog S. cerevisiae antibody
    • DPH2 like 2 antibody
    • DPH2 like 2 S. cerevisiae antibody
    • DPH2-like 2 antibody
    • DPH2_HUMAN antibody
    • DPH2L2 antibody
    • HsDph2 antibody
    • OTTHUMP00000010007 antibody
    • Protein DPH2 homolog antibody
    see all

Images

  • Anti-DPH2 antibody (ab152121) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 52 kDa
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling DPH2 with ab152121 at 1/500 dilution.

References

ab152121 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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