The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
FunctionRequired for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2.
Tissue specificityStrongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes.
Sequence similaritiesBelongs to the DPH1/DPH2 family. DPH2 subfamily.