Overview

  • Product name
  • Description
    Rabbit polyclonal to DPH2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72 - 121 (TTGSKMFILG DTAYGSCCVD VLGAEQAGAQ ALIHFGPACL SPPARPLPVA) of Human DPH2 (NP_001375).

  • Positive control
    • 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • Function
    Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2.
  • Tissue specificity
    Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes.
  • Sequence similarities
    Belongs to the DPH1/DPH2 family. DPH2 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Diphthamide biosynthesis like protein 2 antibody
    • Diphthamide biosynthesis protein 2 antibody
    • Diphthamide biosynthesis protein 2 homolog like 2 antibody
    • Diphthamide biosynthesis protein 2 homolog-like 2 antibody
    • Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 antibody
    • Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 S. cerevisiae antibody
    • DPH 2 antibody
    • DPH 2 homolog antibody
    • DPH2 antibody
    • DPH2 homolog antibody
    • DPH2 homolog S. cerevisiae antibody
    • DPH2 like 2 antibody
    • DPH2 like 2 S. cerevisiae antibody
    • DPH2-like 2 antibody
    • DPH2_HUMAN antibody
    • DPH2L2 antibody
    • HsDph2 antibody
    • OTTHUMP00000010007 antibody
    • Protein DPH2 homolog antibody
    see all

Images

  • Anti-DPH2 antibody (ab82893) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

References

ab82893 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab82893.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up