Overview

  • Product name
    Anti-DPH3 antibody
  • Description
    Rabbit polyclonal to DPH3
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: EDFQYDEDSE TYFYPCPCGD NFSITKEDLE NGEDVATCPS CSLIIKVIYD KDQFVCGETV PAPSANKELV K, corresponding to amino acids 11-81 of Human DPH3.

  • Positive control
    • Human spleen tissue; Human cell line U-2 OS
  • General notes


    ab122265 is mono-specific.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    ab122265 is mono-specific.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in elongation factor 2 and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.
    Down-regulation increases extracellular release of proteoglycans, indicating a possible role in the secretion process. Stimulates binding of GNEFR to SEC5.
  • Tissue specificity
    Widely expressed with highest levels in small intestine, spleen, thymus, heart, liver and lung.
  • Sequence similarities
    Belongs to the DPH3 family.
    Contains 1 DPH-type zinc finger.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSL-type zinc finger-containing protein 2 antibody
    • Deafness locus putative guanine nucleotide exchange factor interacting protein 1 antibody
    • DelGEF-interacting protein 1 antibody
    • DELGIP antibody
    • DelGIP1 antibody
    • DESR1 antibody
    • Diphtheria toxin and Pseudomonas exotoxin A sensitivity required gene 1 antibody
    • DPH3 antibody
    • DPH3 homolog antibody
    • DPH3 KTI11 homolog antibody
    • DPH3_HUMAN antibody
    • DPH3A antibody
    • KTI11 antibody
    • MGC20197 antibody
    • ZCSL2 antibody
    • Zinc finger CSL domain containing 2 antibody
    • Zinc finger CSL type containing 2 antibody
    see all

Images

  • ab122265, at 4µg/ml, staining DPH3 in Human cell line U-2 OS by Immunofluorescence. Antibody staining is shown in green.
  • ab122265, at 1/50 dilution, staining DPH3 in paraffin-embedded Human spleen tissue by Immunohistochemistry.

References

ab122265 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab122265.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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