Overview

  • Product name
    Anti-DPH5 antibody
  • Description
    Mouse polyclonal to DPH5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length DPH5 protein (Human) (NP_001070862.1)

  • Positive control
    • DPH5 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab69347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

Target

  • Relevance
    DPH5 is a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. DPH5 is required for the trimethylation step of diphthamide biosynthesis. There are five named isoforms.
  • Database links
  • Alternative names
    • AD 018 antibody
    • AD018 antibody
    • CGI 30 antibody
    • CGI30 antibody
    • Diphthamide biosynthesis methyltransferase antibody
    • Diphthine synthase antibody
    • DPH5 homolog (S. cerevisiae) antibody
    • NPD015 antibody
    • Protein x 0011 antibody
    see all

Images

  • All lanes : Anti-DPH5 antibody (ab69347) at 1/500 dilution

    Lane 1 : DPH5 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References

ab69347 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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