Overview

  • Product nameAnti-DPM1 antibody [5C5A7]
    See all DPM1 primary antibodies
  • Description
    Mouse monoclonal [5C5A7] to DPM1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment

  • Positive control
    • yeast lysate
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • Purification notesNear homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
  • ClonalityMonoclonal
  • Clone number5C5A7
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

  • FunctionTransfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.
  • PathwayProtein modification; protein glycosylation.
  • Involvement in diseaseDefects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E) [MIM:608799]. CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features.
  • Sequence similaritiesBelongs to the glycosyltransferase 2 family.
  • Cellular localizationEndoplasmic reticulum.
  • Information by UniProt
  • Database links
    • Alternative names
      • CDGIE antibody
      • dolichol monophosphate mannose synthase antibody
      • Dolichol phosphate mannose synthase antibody
      • Dolichol-phosphate mannose synthase antibody
      • Dolichol-phosphate mannosyltransferase antibody
      • Dolichyl phosphate beta D mannosyltransferase antibody
      • dolichyl phosphate mannosyltransferase polypeptide 1 antibody
      • dolichyl phosphate mannosyltransferase polypeptide 1 catalytic subunit antibody
      • Dolichyl-phosphate beta-D-mannosyltransferase antibody
      • DPM synthase antibody
      • DPM1 antibody
      • DPM1_HUMAN antibody
      • Mannose P dolichol synthase antibody
      • Mannose-P-dolichol synthase antibody
      • MPD synthase antibody
      • MPDS antibody
      see all

    Anti-DPM1 antibody [5C5A7] images

    • Anti-DPM1 antibody [5C5A7] (ab113686) + Yeast whole cell lysate at 15 µg

      Predicted band size : 30 kDa

    References for Anti-DPM1 antibody [5C5A7] (ab113686)

    This product has been referenced in:
    • Cartwright BR  et al. Seipin performs dissectible functions in promoting lipid droplet biogenesis and regulating droplet morphology. Mol Biol Cell 26:726-39 (2015). Read more (PubMed: 25540432) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (Saccharomyces cerevisiae)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 30 µg
    Specification Saccharomyces cerevisiae
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"