Overview

  • Product name
  • Description
    Goat polyclonal to DPM1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    RELEVRSPRQNKYS

    , corresponding to N terminal amino acids 15-27 of Human DPM1 (NP_003850.1)

  • Positive control
    • Human liver lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 30 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.
    • Pathway
      Protein modification; protein glycosylation.
    • Involvement in disease
      Defects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E) [MIM:608799]. CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features.
    • Sequence similarities
      Belongs to the glycosyltransferase 2 family.
    • Cellular localization
      Endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDGIE antibody
      • dolichol monophosphate mannose synthase antibody
      • Dolichol phosphate mannose synthase antibody
      • Dolichol-phosphate mannose synthase antibody
      • Dolichol-phosphate mannosyltransferase antibody
      • Dolichyl phosphate beta D mannosyltransferase antibody
      • dolichyl phosphate mannosyltransferase polypeptide 1 antibody
      • dolichyl phosphate mannosyltransferase polypeptide 1 catalytic subunit antibody
      • Dolichyl-phosphate beta-D-mannosyltransferase antibody
      • DPM synthase antibody
      • DPM1 antibody
      • DPM1_HUMAN antibody
      • Mannose P dolichol synthase antibody
      • Mannose-P-dolichol synthase antibody
      • MPD synthase antibody
      • MPDS antibody
      see all

    Images

    • Anti-DPM1 antibody (ab77541) at 0.1 µg/ml + Human liver lysate in RIPA buffer at 35 µg

      Predicted band size : 30 kDa
      Observed band size : 30 kDa

    References

    ab77541 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77541.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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