Overview

  • Product name
  • Description
    Rabbit polyclonal to DPP6
  • Specificity
    This antibody will recognise all four forms of DPP6, but not the other family members.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide based on the stalk region of human DPP6.

    (Peptide available as ab44766.)

Properties

Applications

Our Abpromise guarantee covers the use of ab41809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 98 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      DPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.
    • Cellular localization
      Cell Membrane; single-pass type II membrane protein
    • Database links
    • Alternative names
      • Dipeptidyl aminopeptidase IV related protein antibody
      • Dipeptidyl aminopeptidase like protein 6 antibody
      • Dipeptidyl aminopeptidase related protein antibody
      • Dipeptidyl peptidase 6 antibody
      • Dipeptidyl peptidase IV like protein antibody
      • Dipeptidyl peptidase IV related protein antibody
      • Dipeptidylpeptidase 6 antibody
      • Dipeptidylpeptidase VI antibody
      • DPP VI antibody
      • DPPX antibody
      • VF2 antibody
      see all

    Images

    • All lanes : Anti-DPP6 antibody (ab41809) at 1/1000 dilution

      Lane 1 : ESB
      Lane 2 : Mouse Brain
      Lane 3 : DU145 / IL1-beta Lysate
      Lane 4 : DU145 / IL1-beta CM


      Predicted band size : 98 kDa
      Observed band size : 98 kDa
      Additional bands at : 47 kDa (possible truncated form).

    References

    This product has been referenced in:
    • Sheikh MA  et al. Epigenetic regulation of Dpp6 expression by Dnmt3b and its novel role in the inhibition of RA induced neuronal differentiation of P19 cells. PLoS One 8:e55826 (2013). WB ; Mouse . Read more (PubMed: 23409053) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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