Overview

  • Product nameAnti-DPP8 antibody
    See all DPP8 primary antibodies
  • Description
    Rabbit polyclonal to DPP8
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-39 (MAAAMETEQL GVEIFETADC EENIESQDRP KLEPFYVER) of DPP8 (NP_060213; UniProt ID: Q6V1X1 isoform 4).

  • Positive control
    • WB: A431 whole cell lysate; 293T, H1299 and HepG2 cell lines ICC/IF: HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: for isoform 4, 90 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionDipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function.
  • Tissue specificityUbiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain.
  • Sequence similaritiesBelongs to the peptidase S9B family. DPPIV subfamily.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dipeptidyl peptidase 8 antibody
    • Dipeptidyl peptidase IV-related protein 1 antibody
    • Dipeptidyl peptidase VIII antibody
    • Dipeptidylpeptidase 8 antibody
    • DP8 antibody
    • DPP VIII antibody
    • Dpp8 antibody
    • DPP8_HUMAN antibody
    • DPRP-1 antibody
    • DPRP1 antibody
    • EC 3.4.14.5 antibody
    • MSTP141 antibody
    • Prolyl dipeptidase DPP8 antibody
    see all

Anti-DPP8 antibody images

  • Anti-DPP8 antibody (ab96470) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : for isoform 4, 90 kDa
  • ab96470, at a 1/200 dilution, staining DPP8 in paraformaldehyde fixed HeLa cells by Immunofluorescence.
    Lower image is merged with DNA probe.
  • Anti-DPP8 antibody (ab96470) at 1/1000 dilution + Recombinant human DPP8 protein (ab79662) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

References for Anti-DPP8 antibody (ab96470)

ab96470 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"