Overview

  • Product name
    Anti-DPP8 antibody - Aminoterminal end
    See all DPP8 primary antibodies
  • Description
    Rabbit polyclonal to DPP8 - Aminoterminal end
  • Specificity
    This antibody recognizes all four forms of DPP8. This antibody does not recognize other DPP family members.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to the aminoterminal end of human DPP8.

    (Peptide available as ab44587.)

  • General notes


    This antibody recognizes all four forms of DPP8

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 0.5M Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    This antibody recognizes all four forms of DPP8
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 100 kDa.

Use at 1/1000 when using colourimetric substrates, use at 1/5000 when using chemiluminescent substrates. Dilution optimised using Chromogenic detection.

Target

  • Function
    Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain.
  • Sequence similarities
    Belongs to the peptidase S9B family. DPPIV subfamily.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dipeptidyl peptidase 8 antibody
    • Dipeptidyl peptidase IV-related protein 1 antibody
    • Dipeptidyl peptidase VIII antibody
    • Dipeptidylpeptidase 8 antibody
    • DP8 antibody
    • DPP VIII antibody
    • Dpp8 antibody
    • DPP8_HUMAN antibody
    • DPRP-1 antibody
    • DPRP1 antibody
    • EC 3.4.14.5 antibody
    • MSTP141 antibody
    • Prolyl dipeptidase DPP8 antibody
    see all

References

This product has been referenced in:
  • Dubois V  et al. Dipeptidyl peptidase 9 (DPP9) from bovine testes: identification and characterization as the short form by mass spectrometry. Biochim Biophys Acta 1804:781-8 (2010). WB ; Cow . Read more (PubMed: 20026260) »
  • Dubois V  et al. Enzyme activity and immunohistochemical localization of dipeptidyl peptidase 8 and 9 in male reproductive tissues. J Histochem Cytochem 57:531-41 (2009). Read more (PubMed: 19188489) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Tissue lysate - whole (testis)
Loading amount
40 µg
Specification
testis
Gel Running Conditions
Reduced Denaturing (7,5% gel)
Blocking step
0.05 M Tris buffer containing 0.15 M NaCl, 0.1% Tween 20, 5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Veronique Dubois

Verified customer

Submitted Jul 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (from human liver cDNA library, baculovirus infecte)
Loading amount
0.32 µg
Specification
from human liver cDNA library, baculovirus infecte
Gel Running Conditions
Reduced Denaturing (7,5% gel)
Blocking step
0.05 M Tris buffer containing 0.15 M NaCl, 5 mM NaN3 and 2% Tween 20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Veronique Dubois

Verified customer

Submitted Jun 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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