DPP8 antibody - Catalytic domain (ab42077)

Product Name 

DPP8 antibody - Catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to DPP8 - Catalytic domain

Immunogen 

Synthetic peptide based on the catalytic domain of human DPP8.

(Peptide available as ab44595.)

Reacts with 

Hu, Rat

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Specificity 

This antibody recognises all four forms of DPP8. This antibody does not recognize other DPP family members.

We have a range of domain specific antibodies for this target. For a full list please see all DPP8 antibodies

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/250.
WB: 1/1000 - 1/5000. Predicted molecular weight: 100 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WISH cells, DU145 cells and lysates of LNCaP and Hs1.Tes.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs

Relevance 

DPP-8 (Dipeptidyl Peptidase-8) is a serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP-8 is a member of a broader family of dipeptidyl peptidases including DPP-2, DPP-4, FAP/Seprase, DPP-6, DPP-9, DPP-10, which have differing substrate specificity and tissue localizations. DPP-8 is most closely related to DPP-9, with which it shares 58% identical residues. DPP-8 has only 27% identity with DPP-4, but seems to have similar substrate specificity. Although DPP-4 and FAP-a form a surface-bound heterodimer in some cells, and homodimers in others, DPP-8 seems to be a cytoplasmic protein. The original publication determined that DPP-8 was a monomer, active at neutral pH, and later publications suggest DPP-8 is a homodimer. Substrates include glucagon-like peptide-1, glucagon-like peptide-2, neuropeptide-Y, but with different efficacy relative to DPP-4. DPP-8 has been found in highest abundance in the testis and placenta, but is found in most tissues. Several different splice variants of DPP-8 were seen in the different tissues, with some tissue-specific expression. Four different human sequences are reported to date; 898, 882, 847 and 782 amino acids in length. The longest sequence of human DPP-8 has a predicted mass of 103.4 kDa, and pI of 5.42. The 882, 847 and 782 amino acid forms are predicted at 101.4, 97.5 and 89.9 kDa respectively, with pIs of 5.26, 5.55 and 5.07. Another three partial sequences that are alternatively spliced have deletions in the catalytic domain, and may not be catalytically active. Over expression of DPP-8 caused decreased viability in cells, but a catalytically dead mutant of DPP-8 had the same results, thus some of the DPP-8 activity may be non-enzymatic. Two of the splice variants have longer aminotermini (16 AA insert), and this may affect localization in the cell.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 0.5M Sodium chloride

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for DPP8 antibody - Catalytic domain (ab42077)

  This product has been used in:

Geiss-Friedlander R et al. The cytoplasmic peptidase DPP9 is rate-limiting for degradation of proline-containing peptides. J Biol Chem 284:27211-9 (2009). WB; Human. PubMed: 19667070

If you publish research using ab42077 please let us know so that we can cite the reference on this datasheet.

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