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Western blot - DPP9 antibody - Aminoterminal end (ab42078)
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Western blot - DPP9 antibody - Aminoterminal end (ab42078)
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Product Name
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DPP9 antibody - Aminoterminal end
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See all DPP9 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DPP9 - Aminoterminal end
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Immunogen
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Synthetic peptide corresponding to the amino terminal of Human DPP9.
(Peptide available as ab44597.)
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Reacts with
(species key)
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Hu, Ms, Rat, Cow
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Specificity
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This antibody recognises all three forms of DPP9. This antibody does not recognise other DPP family members.
We have a range of domain specific antibodies for this target. For a full list please see all DPP9 antibodies
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000 - 1/5000. Predicted molecular weight: 98 kDa. Use at 1/1000 with colourimetric substrates, use at 1/5000 with chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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DPP9 is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 0.5M Sodium chloride Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DPP9 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DPP9 antibody - Aminoterminal end images, references, products related to ab42078 and other tools.
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DPP9 antibody - Aminoterminal end images:
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 Western blot - DPP9 antibody - Aminoterminal end (ab42078)
All lanes : DPP9 antibody - Aminoterminal end (ab42078) at 1/1000 dilution
Lane 1 : Cell line DU 145 treated with SF
Lane 2 : Cell line DU 145 treated with RA
Lane 3 : Cell line DU 145 treated with IL1-beta
Lane 4 : Cell line DU 145 treated with SF
Lane 5 : Cell line DU 145 treated with IL1-beta
Lane 6 : Cell line DU 145 treated with Progesterone
Lane 7 : Cell line DU 145 treated with Testosterone
Predicted band size : 98 kDa
Observed band size : 98 kDa
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Western blot - DPP9 antibody - Aminoterminal end (ab42078)
All lanes : DPP9 antibody - Aminoterminal end (ab42078) at 1/5000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Human DPPII protein
Lane 3 : Human DDPIV protein
Lane 4 : Recombinant DPP8 protein
Lane 5 : DPP9 purified protein from Bovine testis
Lane 6 : Molecular weight ladder
Lysates/proteins at 0.32 µg per lane.
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 98 kDa
Observed band size : 106 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes This image is a courtesy of Veronique Dubois
See Abreview
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Search PubMed (MEDLINE) for references to DPP9
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DPP9 antibody - Aminoterminal end - more information
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Customer reviews (feedback) regarding DPP9 antibody - Aminoterminal end |
Customer FAQs regarding DPP9 antibody - Aminoterminal end |
Protocols for DPP9 antibody - Aminoterminal end |
Price and availability of products related to DPP9 antibody - Aminoterminal end |
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DPP9 antibody - Aminoterminal end - related products:
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DPP9 antibody - Aminoterminal end - other tools:
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Contact Abcam with a Technical Enquiry about DPP9 antibody - Aminoterminal end |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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