Ab42080 staining Human normal placenta. Staining is localised to the membrane and cytoplasm. Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody - Catalytic domain (ab42080)This image is a courtesy of an Abreview submitted by Veronique Dubois
ab42080 staining DPP9 in bovine testicular tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Tris/EDTA buffer pH 9.0 and then the sample was blocked with 3% hydrogen peroxide in TBS for 10 minutes at 25°C. The sections were treated for 10 min with 3% H2O2 in TBS to block endogenous peroxidase activity and incubated subsequently for 30 min with 10% nonimmune goat serum to minimize nonspecific antibody binding. The primary antibody (1/50) was incubated with sample in TBS, 0.3% Triton X-100, 0.1 % BSA for 16 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP9 antibody - Catalytic domain (ab42080)This image is courtesy of an Abreview submitted by Denise Yu
ab42080 at 1/50 dilution staining DPP9 in rat brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, permeabilized in 0.2% saponin prior to blocking in 3% H2O2 in PBS for 10 minutes at RT. The sample was incubated with ab42080 for 1 hour. A HRP conjugated goat polyclonal to rabbit Ig, diluted 1/100, was used as the secondary antibody.
ICC/IF image of ab42080 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Tang Z et al. Contribution of upregulated dipeptidyl peptidase 9 (DPP9) in promoting tumoregenicity, metastasis and the prediction of poor prognosis in non-small cell lung cancer (NSCLC). Int J Cancer140:1620-1632 (2017).
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Justa-Schuch D et al. DPP9 is a novel component of the N-end rule pathway targeting the tyrosine kinase Syk. Elife5:N/A (2016).
Read more (PubMed: 27614019) »