Overview

  • Product nameAnti-Dppa2 antibody
    See all Dppa2 primary antibodies
  • Description
    Rabbit polyclonal to Dppa2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (NMEQMEPSVS STSDVKLEKP KKYNPGHLLQ TNEQFTAPQK ARCKIPALPL) of human Dppa2 (NP_620170).

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

Target

  • RelevanceDppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • cancer/testis antigen 100 antibody
    • CT100 antibody
    • Developmental Pluripotency Associated 2 antibody
    • developmental pluripotency-associated gene 2 antibody
    • developmental pluripotency-associated protein 2 antibody
    • ECAT15-2 antibody
    • embryonic stem cell (ESC) associated transcript 15-2 antibody
    • PESCRG1 antibody
    • Pluripotent embryonic stem cell related protein 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 protein antibody
    see all

Anti-Dppa2 antibody images

  • Anti-Dppa2 antibody (ab83043) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

References for Anti-Dppa2 antibody (ab83043)

ab83043 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Permeabilization No
Fixative Formaldehyde
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Verified customer

Submitted Jul 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"